Emmy Gets a BIG GIRL BED!

So for the last few months, Emmy has been asking us for a big girl bed. We were a little reluctant at first but decided it was a good time for her to work towards it- as a goal. She loves sticker charts and is always asking to make them for one thing or another and she is at the age where she totally understands the actual concept and that she has to earn what she wants and if she does what we ask of her, she will get a reward at the end. So my husband and I decided we would make her a sticker chart and once she stayed in her bed a certain number of nights- she would get her big girl bed And low and behold- it worked like a charm. We went through a really rough patch for a while where she would not stay in her bed at all. Every night we would have to bring her back into her room over and over again. We really did not want her to get into the habit that it’s okay and normal to sleep in our bed. For us, that just won’t work. I know for some people it does, but we did not want to get into that.

Anyways- I digress. My point is, the sticker chart worked like a charm and after she filled up the first row, she was able to get her big girl bed. My husband built it and I dressed it all up one day when she as out with her Mimi. The look on her face when she saw her new bed was absolutely PRICELESS. She is so in love with this bed, words can’t even describe it!

So here is her bed (and Emmy) in all her glory! This bed was actually a crib first and with the bed rails, we were able to make it into a full size bed. I plan on her being in this bed until college! Ha! We shall see!
big girl bed
big girl bed
big girl bed
big girl bed

Big Girl Bed- Delta Children |Full Size Bed Rails- Delta Children | Purple Sheet Set- Target | Pom Pom Comforter Set- Target | Mesh Canopy- Pottery Barn Kids | Rug- Lulu & Georgia

 

 

Thank you to Delta Children for sponsoring this post!

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    MyoD, a transcriptional regulator of muscle gene expression and differentiation, is regulated by acetyltransferases and deacetylases .
    A MyoD mutant that cannot be modified by acetylation is
    transcriptionally inactive and fails to promote muscle
    conversion of murine fibroblasts . CONCLUSIONS It is evident that specific forms of sirtuin genes have been extremely conserved all through evolution in a wide spectrum of organisms starting from prokaryotes to mammals.

    Chemical And Organic Elements Of Marine Sponges From The Family Mycalidae

    Tat and SIRT1 coimmunoprecipitate, and, paradoxically, SIRT
    synergistically activates the HIV promoter with Tat.
    Conversely, knockdown of SIRT1 via small interfering RNAs inhibits Tatmediated transactivation of the HIV lengthy terminal repeat.

    Treatment with a splitomicin analog that specifically inhibits SIRT1 also
    inhibits Tat-mediated transactivation . Tat transactivation is
    defective in SIRT1-null mouse embryonic fibroblasts and may
    be rescued by expression of SIRT1.

    Histone Acetyltransferase And Leukaemogenic Fusion Proteins

    SIRT2 interacts with the homeobox transcription issue HOXA10 in vitro and in vivo and should modulate its transcriptional activity .
    HOXA10 plays a role in myeloid and B-lymphoid progenitor cell differentiation and within the adult uterus throughout periimplantation events .

    HOX proteins are evolutionarily conserved transcription factors that
    play pivotal roles in developmental regulation via pattern formation. This regulation is mediated via
    the homeodomain, a conserved protein motif that’s responsible
    for sequence-particular DNA binding . Differential cofactor binding to HOX proteins can modify their actions from activation to repression .
    Tumor Suppressor The gene for SIRT2 is situated at chromosome 19q13.2, a area incessantly deleted in human gliomas .

    Interestingly, somewhat than repressing runx2 activity by way of
    the anticipated mechanism of histone deacetylation, HDAC4 seems instead to inhibit the DNA
    binding of Runx2. This mannequin is supported by the discovering that a construct of only the first 220 amino acids of HDAC4 represses runx2mediated transcription nearly as successfully as the complete-size HDAC4.
    However, the mannequin is weakened by the finding that
    a construct consisting of just the C-terminal catalytic domain of HDAC4
    is also a significant repressor. Clearly, that is an thrilling new chapter in the biology of sophistication IIa HDACs.
    INTRODUCTION The directed acetylation and deacetylation of lysine
    residues in the N-terminal tails of histone octamer subunits provide a rapid, reversible means for the modification of gene
    expression ranges. In all recognized circumstances, removing of acetyl teams
    from histones by histone deacetylases represses gene transcription at
    the affected loci, and the addition of acetyl groups activates transcription.

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    A mannequin is proposed that involves a role for SIRT1 within the recycling of Tat to its unacetylated state, a type of Tat that’s essential
    to initiate its transcriptional activity on the HIV promoter
    . BIOLOGY OF SIRT1 Transcriptional Regulation by SIRT1 SIRT1 immediately deacetylates K16 of histone H4 and K9 of histone H3, and inhibition of SIRT1 expression leads to hyperacetylation of both residues .

    Acetylated K16 is a mark for euchromatin, whereas heterochromatin is hypoacetylated in K16 .
    SIRT1 also interacts with histone H1, deacetylates its K26, and results in heterochromatin formation .
    Several transcription components appear to target SIRT1
    to chromatin. Chromatin immunoprecipitation experiments indicate that SIRT1 is certain to the promoters of genes concerned in skeletal
    muscle differentiation . There, SIRT1 probably mediates the deacetylation of K9 and K14 of histone H3, the identical residues that are focused by SIRT1 in vitro .

    Optimistic Effects

    Runx1 appears to be important for hematopoietic improvement, and runx3-poor mice have defects in limb improvement and the
    digestive system (18–20). Both molecules play essential roles within the developmental regulation of the CD4
    and CD8 T-cell coreceptors . Mice poor in Runx 2 show a profound
    defect in the calcification of bone tissue owing to a block in osteoblast differentiation .
    Strikingly, the just lately revealed phenotype of HDAC4-poor mice turns out to involve a untimely calcification of
    bone tissue, as could be anticipated from a Runx2 gain-of-function mutation .

    Transcription elements such as p53, E2F1,
    and GATA1 are at present thought to depend on acetylation to advertise binding to
    focus on DNA. Inhibition of deacetylases which have enzymatic exercise in opposition to the
    acetylated form of p53 will increase p21 levels to hinder cell cycle development, whereas exercise towards the deacetylation of E2F1 could promote retinoblastoma protein -mediated differentiation .
    Additionally, HDAC inhibition permits the continued acetylated
    state of proteins, corresponding to TCF in Drosophila and ACTR, disrupting protein-protein interplay
    with its companion armadillo and nuclear receptors, respectively .
    Furthermore, there is now proof that shows the importance
    of reversible acetylation in regulating issue localization and signaling.
    Chen et al. present evidence that acetylated NF-κB possesses prosurvival transcription proficiency.
    They propose that inhibitor κB (IκB), NF-κB’s inhibitory partner,
    fails to bind to the acetylated form of NF-κB, blocking IκB-mediated transport of the complex out of
    the nucleus. Transcriptional Regulation and HOXA10 The nuclear-cytoplasmic shuttling
    of SIRT2 means that it could even have nuclear activities.

    This conservation of different types of sirtuins most likely pertains to preserving binding interactions with other proteins
    which might be related to sirtuins in multiprotein complexes and binding interactions with specific acetylated protein substrates.
    Therefore research that reveal the operate of sirtuins
    in each prokaryotic and primitive eukaryotic methods are likely to be very useful in elucidating the operate of mammalian sirtuins and
    vice versa. Remarkable proof of this kind of conservation of perform across a wide evolutionary time
    span was discovered just lately in regard to
    similarities between SIRT1-containing chromatin-modifying complexes found in Drosophila and human cells.
    The Drosophila larval complex contained a number of proteins including SIRT1 and
    Enhancer of Zeste . As sirtuins proceed to be studied in many numerous prokaryotic
    and eukaryotic model techniques, additional extensively relevant insights into the features of those highly conserved proteins can be anticipated.
    Runx2 The three members of the Runx family of transcription elements in mammals share homology throughout most
    of their sequences and in their N-terminal DNA binding domains to the Drosophila transcription issue
    Runt. The Runx relations transcriptionally regulate all kinds of goal genes and might function as both activators or
    repressors of transcription (reviewed in ref. 17).

    Class IIa HDACs, which include HDACs 4, 5, 7, and 9, are outlined by a big, functionally
    important noncatalytic N-terminal area. This area mediates each the recruitment of class IIa HDACs to specific promoters and the sign-dependent shuttling of the category IIa HDACs between the nucleus and the
    cytoplasm.

    These HDAC inhibitors played important roles not only in biochemical analyses of the role of histone acetylation but also
    in cloning the HDAC cDNA .

    Both acetylation and deacetylation of histones are catalyzed by histone acetyltransferases and histone deacetylases , respectively.

    Histone acetylation appears to constitute a sign which will function together with other
    covalent modifications to generate an epigenetic code.

    This specific sign is reversed by a mechanism involving HDACs, which have been found as transcriptional regulators associated to lowered potassium dependency 3 yeast ,
    histone deacetylase-A1 , and silent data regulator 2 .

    The mixture of these two functionalities in the N-terminal domain of sophistication IIa HDACs defines them as
    sign-dependent repressors of particular sets of genes.
    Thus they’ve essential roles in the developmentally
    regulated expression of genes that are involved in the differentiation and
    performance of muscle, immune, and neural cells. Because of
    the intensive functional characterization of their shared N-terminal domain and also the quickly emerging phenotypic data from mutant animals, class IIa HDACS
    are maybe the most effective understood of the HDACs.
    The function of this chapter is to outline what’s presently known concerning the structure, function, phylogeny, and regulation of this
    family of versatile, essential developmental gene regulators.
    In but one instance of such an approach, HDAC inhibitors are currently being tested in human medical trials for most cancers, and therapeutic profit and tolerability have been reported.

    Conversely, overexpression of HDAC4 in creating bone produces a phenotype just like
    that of Runx2 deficiency. The authors of this research hypothesized that HDAC4 might
    due to this fact function as a corepressor of runx2.
    They proceed to reveal this, displaying that the N-terminal Runt area of runx2 interacts
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    We should all be aware of the pressure that our children cope
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    Morgentaler does admit that some males could require testosterone
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    Chronic pain is mostly felt within the upper arm and the front and back of the shoulder.

    “It gets tough to lift your arm and reach round to your again” says Dr.
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    A bony ball at the top of the upper arm bone suits right into a shallow socket within the shoulder blade
    . Four muscle tissue which are known as the rotator cuff encompass
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    Advancements in implant design and extra cautious patient choice have improved outcomes and longevity of shoulder substitute lately.

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    Antibiotics are not routinely beneficial in conjuction with corticosteroids within the administration of the in any other case healthy affected person 12.

    Monitor intently to detect the event of significant side effects.
    For occasion, the brochure for Bayer’s YAZ mentions adjustments within the endometrial effects that reduce the
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    Such a secondary mechanism would occur if
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    four) Drug interactions with active pills that decrease contraceptive estrogen or progestogen ranges.

    Seriously because it has testosterone in it, I can have a
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    In reality, based on an article revealed by Harvard Health Publications, some doctors
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    In the best phrases, the second half of the protocol is to
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    sufferers entered into the trial, fifty six have been included in the evaluation. Three patients
    who obtained aminoglutethimide achieved a partial response (11%),
    whereas one partial and 5 full responses (21%) were
    reported in the hydrocortisone group.
    COCPs provide efficient contraception from the very
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    The failure of the testes to reply to an HCG challenge is indicative of
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    In the practical sort of hypogonadism, the power to stimulate the HPTA to pro­duce LH and
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    “I seemed higher at forty than I did at 20, so a lot of guys assumed I was on something,” he says.
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    I found this article very interesting…please read!

    Do you remember the blockbuster hit film The Matrix that was released in 1999? You may not know this, but it has deep spiritual implications concerning the times we are living in and Bible prophecy.

    It tells a story of how these “agents” are trying to turn us into machines. We are closer then ever before for this to become a reality when they cause us to receive an implantable microchip in our body during a time when physical money will be no more.

    You may have seen on NBC news concerning the implantable RFID microchip that some people are getting put in their hand to make purchases, but did you know this microchip matches perfectly with prophecy in the Bible?

    “He (the false prophet who deceives many by his miracles) causes all, both small and great, rich and poor, free and slave, to receive a mark on their right hand or on their foreheads, and that no one may buy or sell except one who has the mark or the name of the beast, or the number of his name…

    You also may have heard of the legendary number “666” that people have been speculating for possibly thousands of years on what it actually means. This article shares something I haven’t seen before, and I don’t think there could be any better explanation for what it means to calculate 666. This is no hoax. Very fascinating stuff!

    …Here is wisdom. Let him who has understanding calculate the number of the beast, for it is the number of a man: His number is 666″ (Revelation 13:16-18 NKJV).

    To see all the details showing why the Bible foretold of all these things, check out this article!

    Article: https://biblewoke.com/rfid-mark-of-the-beast-666-revealed

    GOD is sending out His end time warning:

    “Then a third angel followed them, saying with a loud voice, ‘If anyone worships the beast and his image, and receives his mark on his forehead or on his hand, he himself shall also drink of the wine of the wrath of God, which is poured out full strength into the cup of His indignation. He shall be tormented with fire and brimstone in the presence of the holy angels and in the presence of the Lamb. And the smoke of their torment ascends forever and ever; and they have no rest day or night, who worship the beast and his image, and whoever receives the mark of his name'” (Revelation 14:9-11).

    In the Islamic religion they have man called the Mahdi who is known as their messiah of whom they are waiting to take the stage. There are many testimonies from people online who believe this man will be Barack Obama who is to be the biblical Antichrist based off dreams they have received. I myself have had strange dreams about him like no other person. So much so that I decided to share this information.

    He came on stage claiming to be a Christian with no affiliation to the Muslim faith…

    “In our lives, Michelle and I have been strengthened by our Christian faith. But there have been times where my faith has been questioned — by people who don’t know me — or they’ve said that I adhere to a different religion, as if that were somehow a bad thing,” – Barack Obama

    …but was later revealed by his own family members that he indeed is a devout Muslim.

    So what’s in the name? The meaning of someones name can say a lot about a person. God throughout history has given names to people that have a specific meaning tied to their lives. How about the name Barack Obama? Let us take a look at what may be hiding beneath the surface…

    “And He (Jesus) said to them (His disciples), ‘I saw Satan fall like lightning from heaven'” (Luke 10:18).

    In the Hebrew language we can uncover the meaning behind the name Barack Obama.

    Barack, also transliterated as Baraq, in Hebrew is: lightning

    baraq – Biblical definition:

    From Strongs H1299; lightning; by analogy a gleam; concretely a flashing sword: – bright, glitter (-ing, sword), lightning. (Strongs Hebrew word H1300 baraq baw-rawk’)

    Barak ‘O’bamah, The use of bamah is used to refer to the “heights” of Heaven.

    bamah – Biblical definition:

    From an unused root (meaning to be high); an elevation: – height, high place, wave. (Strongs Hebrew word H1116 bamah baw-maw’)

    The day following the election of Barack Obama (11/04/08), the winning pick 3 lotto numbers in Illinois (Obama’s home state) for 11/5/08 were 666.

    Obama was a U.S. senator for Illinois, and his zip code was 60606.

    Seek Jesus while He may be found…repent, confess and forsake your sins and trust in the savior! Jesus says we must be born again by His Holy Spirit to enter the kingdom of God…God bless!

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